Soil-borne diseases are a major constraint for the Australian vegetable industry. This project developed DNA based tests for quantifying key pathogens and evaluated their use as a tool for pre-plant risk assessment, monitoring of inoculum levels, and symptom testing. The DNA tests are available through SARDI’s PREDICTA research service. Focus of DNA test development and validation was clubroot, pathogens causing cavity spot, root knot and root lesion nematodes in order to develop testing services of relevance to brassica and carrot growers.